Porcine Reproductive and Respiratory Syndrome Virus
(PRRSV) Antibody ELISA kit
- Brief
PRRSV occurs in most major pig-producing areas throughout the world. The reproductive failure is
characterised by abortions, stillbirths, and the birth of weak piglets that often die soon after birth of
respiratory disease and secondary infections. Older pigs may demonstrate mild signs of
respiratory disease, sometimes complicated by secondary infections. Animals other than pigs do
not seem to be affected by PRRSV. - Usage
The porcine reprodutive and respiratory syndrome virus (PRRSV) ELISA test kit is used for the
detection of PRRSV antibodies in swine serum; assessment of immunity conditions against porcine
reprodutive and respiratory syndrome virus in the pig farm; and investigation epidemiology of the
PRRSV. - Principle
The test kit is the antigen coated microplate made through the inactivation, purification and
concentration of the PRRSV. In the test, the diluted control serum and sample are added, then
incubate. If porcine PRRSV specific antibodies exist in the sample, it will be bound with the
antigens on the microplate, remove the unbound antibodies and other components by washing.
Add IgG-HRP to specifically bind with complex of antibody-antigens on the microplate. Then the
unbound enzyme conjugate (Anti-pig IgG-HRP combination) will be removed by washing. Then add
the TMB substrate to the wells, react with HRP to generate a blue color. At last, end the reaction by
adding stop solution, read the absorbance value at 630 nm.
