Aminohydantion (AHD) ELISA Test Kit
- Principle
This test kit is based on the competitive enzyme immunoassay for the detection of
Aminohydantion (AHD) in the sample. The coupling antigens are pre-coated on the micro-well
stripes. The Aminohydantion (AHD) in the sample and the coupling antigens pre-coated on the
micro-well stripes compete for the anti-AHD antibodies. After the addition of the enzyme conjugate,
the TMB substrate is added for coloration. The optical density (OD) value of the sample has a
negative correlation with the AHD in it. This value is compared to the standard curve and the AHD
concentration is subsequently obtained. - Technical specifications
Sensitivity: 0.05 ppb
Detection limit
Tissue and honey················································································· 0.1 ppb
Shrimp and fish (some interference in shrimp and fish)·····················0.15 ppb
Recovery rate
Shrimp and fish··················································································· 90±15%
Honey, meat, liver··············································································· 80±15%
Cross-reaction rate
AHD········································································································· 100%
AMOZ··································································································· ﹤0.1%
AOZ······································································································ ﹤0.1%
SEM······································································································﹤0.1%
