Product Name
Fecal Occult Blood(FOB)Test Kit (Colloidal Gold Method)
Purpose
To detect the content of FOB in human fecal in vitro qualitatively.For Professional Use only.
Principles of Detection
The FOB Rapid Test is a one-step chromatographic sandwich immunoassay. The test consists of: 1) a conjugate pad containing HB monoclonal antibody conjugated with colloidal gold. 2) a nitrocellulose membrane strip containing a test line (T line) and a control line (C line). The T line is pre-coated with another HB antibody, and the C line is pre-coated with a control line antibody (goat-anti-mouse IgG antibody).
After addition of the sample, FOB will bind with a colloidal gold-labeled antibody on the conjugate release pad. The resulting complex flows over the nitrocellulose membrane where a specific capture reagent is pre-coated and a red line can be seen at the test zone (T). Unreacted colloidal gold-labeled antibodies in the sample are captured at the control zone (C). The intensity and speed at which the color develops depend on the FOB concentration in the sample.
Storage conditions and shelf life
Store in a sealed aluminum foil bag at 2~30°C.Forbidden freezing.
Shelf life 18 months.
The test card should be used within 1hour after unpacked to avoid disable in the air ,please use it immediately.
Test procedure
Read the complete instruction before the test
- Return the kit to room temperature before use.
- Tear the bag, take out the test card, place the test card on a horizontal table for testing, and use it within 1 hour.
- Take out the sample processing tube, collect an appropriate amount of fecal specimen (20-50 mg) into the sample processing tube with sampling cotton tip, mix thoroughly, add 50ul(2 drops) into the hole of test card, and read the results after 5 minutes.And the observation results after 20 minutes were invalid
Reference range
This kit can detect FOB at a minimum of 50ng/ml.
Performance Characteristics
- Critical value and repeatability:
1) The quality control substance of FOB with a concentration of 50ug/mL was detected for 20 times, and the positive coincidence rate was ≥95%, and the color uniformity, and the reaction results were consistent.
2) The quality control substance of FOB with a concentration of 10ng/mL was detected for 20 times, and the negative coincidence rate was ≥95%, and the color uniformity, and the reaction results were consistent. - Analysis specificity: 500ug/mL bovine HB,500ug/mL pork HB,500ug/ mL chicken HB,500ug/mL goat HB,2000ug/mL Horseradish Peroxidase specific quality control substance were all negative.
3.HOOK effect: human FOB positive quality control substance of 2000ug/mL was detected and the result was positive.
